Shimadzu Spd20a User Manual Better (2025)
: Capable of simultaneous detection at two different wavelengths, which is critical for identifying peaks via absorbance ratios or monitoring compounds with different absorption maxima. Technical Specifications UV-VIS detector / Photodiode Array Detector - Shimadzu
Which are you using to operate the detector (e.g., LabSolutions, Empower, Chromeleon)?
Use the deuterium lamp’s natural emission line at 656.1 nm (visible range). In [DIAG] → WAVE CHECK , the detector auto-verifies against this line. If deviation >2 nm, service is required.
~2000 hours. The detector tracks this ( [FUNC] → [DIAG] → LAMP USED TIME ). Replace when:
190 to 700 nm (SPD-20A) or 190 to 900 nm (SPD-20AV). Sensitivity: Noise level of AU (Absorbance Units). Linearity: Wide linearity up to 2.5 AU. shimadzu spd20a user manual better
Fix: Verify the UV-Vis absorbance spectrum of your target compound. The internal mechanical shutter is stuck.
Maximizing Your HPLC Performance: A Guide to the Shimadzu SPD-20A Go to product viewer dialog for this item. /20AV User Manual and Beyond The and Go to product viewer dialog for this item.
The instrument will automatically check the ROM/RAM, wavelengths, and optical alignment. Wait for the initialization screen to pass.
The flow cell is the heart of the detector. Contamination, bubbles, or improper sealing causes significant issues. : Capable of simultaneous detection at two different
features a highly sensitive optical unit designed for low noise and high stability.
Shimadzu provides two complementary manuals:
Baseline spikes, erratic noise, or pressure fluctuation.
Adjust the (typically 10 Hz or 20 Hz depending on your peak widths). In [DIAG] → WAVE CHECK , the detector
Maximizing Performance: Making the Shimadzu SPD-20A User Manual Better for You
Allow users to instantly identify the detector's state using the front panel indicator. Key Indicators: Green: System ready. Orange: Sleep mode. Red: Active error requiring troubleshooting. "D2" on Display: Deuterium lamp is successfully powered on. 2. Routine Maintenance Interval Tracker
The manual suggests cleaning the flow cell with solvent. But every HPLC chemist knows the horror of a clogged or dirty cell causing massive baseline drift.
